Journal: Respiratory Research

Article Title: Whole genome comparisons reveal gut-to-lung translocation of Escherichia coli and Burkholderia cenocepacia in two cases of ventilator-associated pneumonia in ICU patients

doi: 10.1186/s12931-025-03204-x

Figure Lengend Snippet: Immunological changes and gut integrity in P. aeruginosa -induced lung infection mouse. ( A - C ) Representative flow cytometry plots and proportions of Tregs identified as FoxP3 + within CD3 + CD4 + CD45 + T cells in the spleen ( A ), MLNs ( B ), and colonic lamina propria ( C ) across experimental groups ( n = 3). ( D ) Representative flow cytometry plots and proportions of Th17 cells identified as IL-17 A + within CD3 + CD4 + CD45 + cells in the spleen across groups ( n = 3). ( E ) Representative H&E-stained colon sections from Cont, Ec, Pa, and EcPa groups. Scale bars = 50 μm ( n = 5). ( F ) Disease activity index scores indicating colon injury across groups ( n = 5). ( G ) Histological score of colon injury from H&E-stained sections ( n = 5). ( H ) Submucosa thickness (µm) of the colon in each group ( n = 5). Statistics by one-way ANOVA with Tukey’s post-hoc test. Values shown are the mean ± SD. * p < 0.05. ** p < 0.01

Article Snippet: Surface markers FITC-anti-mouse CD3 (Thermo Fisher Scientific, 11-0032-80, USA), Percp-anti-mouse CD4 (BioLegend, 100537, USA), and APC-anti-mouse CD45 (BioLegend, 147707, USA) were used, along with the intranuclear staining marker PE-anti-mouse Foxp3 (Miltenyi Biotec, 130-111-678, Germany).

Techniques: Infection, Flow Cytometry, Staining, Activity Assay